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Profiling molecular changes induced by hydrogen treatment of lung allografts prior to procurement.

ABSTRACT: We previously demonstrated that donor treatment with inhaled hydrogen protects lung grafts from cold ischemia/reperfusion (I/R) injury during lung transplantation. To elucidate the mechanisms underlying hydrogen's protective effects, we conducted a gene array analysis to identify changes in gene expression associated with hydrogen treatment.Donor rats were exposed to mechanical ventilation with 98% oxygen and 2% nitrogen or 2% hydrogen for 3 h before harvest; lung grafts were stored for 4h in cold Perfadex. Affymetrix gene array analysis of mRNA transcripts was performed on the lung tissue prior to implantation.Pretreatment of donor lungs with hydrogen altered the expression of 229 genes represented on the array (182 upregulated; 47 downregulated). Hydrogen treatment induced several lung surfactant-related genes, ATP synthase genes and stress-response genes. The intracellular surfactant pool, tissue adenosine triphosphate (ATP) levels and heat shock protein 70 (HSP70) expression increased in the hydrogen-treated grafts. Hydrogen treatment also induced the transcription factors C/EBP? and C/EBP?, which are known regulators of surfactant-related genes.Donor ventilation with hydrogen significantly increases expression of surfactant-related molecules, ATP synthases and stress-response molecules in lung grafts. The induction of these molecules may underlie hydrogen's protective effects against I/R injury during transplantation.

SUBMITTER: Tanaka Y

PROVIDER: S-EPMC4007057 | biostudies-other | 2012 Sep

REPOSITORIES: biostudies-other

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Profiling molecular changes induced by hydrogen treatment of lung allografts prior to procurement.

Tanaka Yugo Y Shigemura Norihisa N Kawamura Tomohiro T Noda Kentaro K Isse Kumiko K Stolz Donna Beer DB Billiar Timothy R TR Toyoda Yoshiya Y Bermudez Christian A CA Lyons-Weiler James J Nakao Atsunori A

Biochemical and biophysical research communications 20120807 4

<h4>Background</h4>We previously demonstrated that donor treatment with inhaled hydrogen protects lung grafts from cold ischemia/reperfusion (I/R) injury during lung transplantation. To elucidate the mechanisms underlying hydrogen's protective effects, we conducted a gene array analysis to identify changes in gene expression associated with hydrogen treatment.<h4>Methods</h4>Donor rats were exposed to mechanical ventilation with 98% oxygen and 2% nitrogen or 2% hydrogen for 3 h before harvest; l ...[more]

PMID: 22902635

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Dataset Information

Profiling molecular changes induced by hydrogen treatment of lung allografts prior to procurement.

Publications

Profiling molecular changes induced by hydrogen treatment of lung allografts prior to procurement.

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