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Labeled Ligand Displacement: Extending NMR-Based Screening of Protein Targets.

ABSTRACT: NMR spectroscopy has enjoyed widespread success as a method for screening protein targets, especially in the area of fragment-based drug discovery. However, current methods for NMR-based screening all suffer certain limitations. Two-dimensional methods like "SAR by NMR" require isotopically labeled protein and are limited to proteins less than about 50 kDa. For one-dimensional, ligand-based methods, results can be confounded by nonspecific compound binding, resonance overlap, or the need for a special NMR probe. We present here a ligand-based method that relies on the exchange broadening observed for a (13)C-labeled molecule upon binding to a protein target (labeled ligand displacement). This method can be used to screen both individual compounds and mixtures and is free of the artifacts inherent in other ligand-based methods.

SUBMITTER: Swann SL

PROVIDER: S-EPMC4007843 | biostudies-other | 2010 Sep

REPOSITORIES: biostudies-other

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Labeled Ligand Displacement: Extending NMR-Based Screening of Protein Targets.

Swann Steven L SL Song Danying D Sun Chaohong C Hajduk Philip J PJ Petros Andrew M AM

ACS medicinal chemistry letters 20100624 6

NMR spectroscopy has enjoyed widespread success as a method for screening protein targets, especially in the area of fragment-based drug discovery. However, current methods for NMR-based screening all suffer certain limitations. Two-dimensional methods like "SAR by NMR" require isotopically labeled protein and are limited to proteins less than about 50 kDa. For one-dimensional, ligand-based methods, results can be confounded by nonspecific compound binding, resonance overlap, or the need for a s ...[more]

PMID: 24900211

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