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Down-regulation of Sox11 is required for efficient osteogenic differentiation of adipose-derived stem cells.

ABSTRACT: Adipose-derived stem cells represent a type of mesenchymal stem cells with the attendant capacity to self-renew and differentiate into multiple cell lineages. We have performed a microarray-based gene expression profiling of osteogenic differentiation and found that the transcription factor Sox11 is down-regulated during the process. Functional assays demonstrate that down-regulation of Sox11 is required for an efficient differentiation. Furthermore, results from forced expression of constitutively-active and dominant-negative derivatives of Sox11 indicate that Sox11 functions as a transcriptional activator in inhibiting osteogenesis. Sox11 thus represents a novel regulator of osteogenesis whose expression and activity can be potentially manipulated for controlled differentiation.

SUBMITTER: Choi MK

PROVIDER: S-EPMC4012083 | biostudies-other | 2014 Apr

REPOSITORIES: biostudies-other

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Down-regulation of Sox11 is required for efficient osteogenic differentiation of adipose-derived stem cells.

Choi Mi Kyung MK Seong Ikjoo I Kang Seon Ah SA Kim Jaesang J

Molecules and cells 20140407 4

Adipose-derived stem cells represent a type of mesenchymal stem cells with the attendant capacity to self-renew and differentiate into multiple cell lineages. We have performed a microarray-based gene expression profiling of osteogenic differentiation and found that the transcription factor Sox11 is down-regulated during the process. Functional assays demonstrate that down-regulation of Sox11 is required for an efficient differentiation. Furthermore, results from forced expression of constitutiv ...[more]

PMID: 24722414

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| S-EPMC8258422 | biostudies-literature

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| S-EPMC3938923 | biostudies-literature

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