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Z-DNA-forming sites within the human beta-globin gene cluster.

ABSTRACT: Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation.

SUBMITTER: Muller V

PROVIDER: S-EPMC40132 | biostudies-other | 1996 Jan

REPOSITORIES: biostudies-other

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Z-DNA-forming sites within the human beta-globin gene cluster.

Müller V V Takeya M M Brendel S S Wittig B B Rich A A

Proceedings of the National Academy of Sciences of the United States of America 19960101 2

Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, ...[more]

PMID: 8570634

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