Z-DNA-forming sites within the human beta-globin gene cluster.
Ontology highlight
ABSTRACT:
SUBMITTER: Muller V
PROVIDER: S-EPMC40132 | biostudies-other | 1996 Jan
REPOSITORIES: biostudies-other
Publications
Z-DNA-forming sites within the human beta-globin gene cluster.
Müller V V Takeya M M Brendel S S Wittig B B Rich A A
Proceedings of the National Academy of Sciences of the United States of America 19960101 2
Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, ...[more]
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