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An antisense transcript from the Xenopus laevis bFGF gene coding for an evolutionarily conserved 24 kd protein.

ABSTRACT: Screening of a Xenopus laevis oocyte cDNA library with a rat basic fibroblast growth factor (bFGF) cDNA led to the isolation of a 1.35 kb sequence containing exon III of the bFGF gene. Reverse complementary listing of this sequence revealed a polyadenylated transcript with an open reading frame coding for an unknown protein of mol. wt 24,292 daltons. The coding part of bFGF exon III is located in this putative mRNA in opposite direction within the 3' untranslated region. By hybridization studies on transcription orientation with single-stranded probes it could be proven that this transcript actually represents an antisense transcript to part of the Xenopus bFGF gene. Sequence organization on corresponding genomic fragments revealed that it is processed from a larger precursor by splicing mechanisms. Sequence comparison with elongated transcripts from the bFGF gene in human hepatoma has shown that the gene coding for the antisense mRNA is evolutionarily conserved.

SUBMITTER: Volk R

PROVIDER: S-EPMC401371 | biostudies-other | 1989 Oct

REPOSITORIES: biostudies-other

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An antisense transcript from the Xenopus laevis bFGF gene coding for an evolutionarily conserved 24 kd protein.

Volk R R Köster M M Pöting A A Hartmann L L Knöchel W W

The EMBO journal 19891001 10

Screening of a Xenopus laevis oocyte cDNA library with a rat basic fibroblast growth factor (bFGF) cDNA led to the isolation of a 1.35 kb sequence containing exon III of the bFGF gene. Reverse complementary listing of this sequence revealed a polyadenylated transcript with an open reading frame coding for an unknown protein of mol. wt 24,292 daltons. The coding part of bFGF exon III is located in this putative mRNA in opposite direction within the 3' untranslated region. By hybridization studies ...[more]

PMID: 2479540

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Project description:Decades of research identify the hippocampal formation as central to memory storage and recall. Events are stored via distributed population codes, the parameters of which (e.g., sparsity and overlap) determine both storage capacity and fidelity. However, it remains unclear whether the parameters governing information storage are similar between species. Because episodic memories are rooted in the space in which they are experienced, the hippocampal response to navigation is often used as a proxy to study memory. Critically, recent studies in rodents that mimic the conditions typical of navigation studies in humans and nonhuman primates (i.e., virtual reality) show that reduced sensory input alters hippocampal representations of space. The goal of this study was to quantify this effect and determine whether there are commonalities in information storage across species. Using functional molecular imaging, we observe that navigation in virtual environments elicits activity in fewer CA1 neurons relative to real-world conditions. Conversely, comparable neuronal activity is observed in hippocampus region CA3 and the dentate gyrus under both conditions. Surprisingly, we also find evidence that the absolute number of neurons used to represent an experience is relatively stable between nonhuman primates and rodents. We propose that this convergence reflects an optimal ensemble size for episodic memories.SIGNIFICANCE STATEMENT One primary factor constraining memory capacity is the sparsity of the engram, the proportion of neurons that encode a single experience. Investigating sparsity in humans is hampered by the lack of single-cell resolution and differences in behavioral protocols. Sparsity can be quantified in freely moving rodents, but extrapolating these data to humans assumes that information storage is comparable across species and is robust to restraint-induced reduction in sensory input. Here, we test these assumptions and show that species differences in brain size build memory capacity without altering the structure of the data being stored. Furthermore, sparsity in most of the hippocampus is resilient to reduced sensory information. This information is vital to integrating animal data with human imaging navigation studies.

Dataset Information

An antisense transcript from the Xenopus laevis bFGF gene coding for an evolutionarily conserved 24 kd protein.

Publications

An antisense transcript from the Xenopus laevis bFGF gene coding for an evolutionarily conserved 24 kd protein.

Similar Datasets

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