Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay.
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ABSTRACT:
SUBMITTER: Shoemaker CJ
PROVIDER: S-EPMC4022135 | biostudies-other | 2010 Oct
REPOSITORIES: biostudies-other
Publications
Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay.
Shoemaker Christopher J CJ Eyler Daniel E DE Green Rachel R
Science (New York, N.Y.) 20101001 6002
No-go decay (NGD) is one of several messenger RNA (mRNA) surveillance systems dedicated to the removal of defective mRNAs from the available pool. Two interacting factors, Dom34 and Hbs1, are genetically implicated in NGD in yeast. Using a reconstituted yeast translation system, we show that Dom34:Hbs1 interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off. Our data further indicate that these recycling activities are shared by the homologous translation terminati ...[more]
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