Ontology highlight
ABSTRACT:
SUBMITTER: Sarkar B
PROVIDER: S-EPMC4030794 | biostudies-other | 2014 May
REPOSITORIES: biostudies-other
ACS chemical neuroscience 20140331 5
Dopaminergic neurotransmission has been investigated extensively, yet direct optical probing of dopamine has not been possible in live cells. Here we image intracellular dopamine with sub-micrometer three-dimensional resolution by harnessing its intrinsic mid-ultraviolet (UV) autofluorescence. Two-photon excitation with visible light (540 nm) in conjunction with a non-epifluorescent detection scheme is used to circumvent the UV toxicity and the UV transmission problems. The method is established ...[more]