Unknown

Dataset Information

0

Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica.


ABSTRACT: In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn(2+) concentrations. We establish that Mn(2+) accumulates intracellularly on injection into the living Aplysia and that its concentration increases when the animals are presented with a sensory stimulus. We also show that we can distinguish between neuronal activities elicited by different types of stimuli. This method opens up a new avenue into probing the functional organization and plasticity of neuronal networks involved in goal-directed behaviors with single-cell resolution.

SUBMITTER: Radecki G 

PROVIDER: S-EPMC4060695 | biostudies-other | 2014 Jun

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC4461915 | biostudies-literature
| S-EPMC5647638 | biostudies-literature
| S-EPMC4028319 | biostudies-literature
| S-EPMC4153062 | biostudies-literature
| S-EPMC9396074 | biostudies-literature
| PRJNA201440 | ENA
| PRJNA38661 | ENA
| PRJNA104537 | ENA
| PRJNA412022 | ENA
| PRJNA111983 | ENA