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Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica.

ABSTRACT: In this work, we show the feasibility of performing functional MRI studies with single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic resonance microscopy allows the identification of most motor neurons in the buccal network of Aplysia at low, nontoxic Mn(2+) concentrations. We establish that Mn(2+) accumulates intracellularly on injection into the living Aplysia and that its concentration increases when the animals are presented with a sensory stimulus. We also show that we can distinguish between neuronal activities elicited by different types of stimuli. This method opens up a new avenue into probing the functional organization and plasticity of neuronal networks involved in goal-directed behaviors with single-cell resolution.

SUBMITTER: Radecki G

PROVIDER: S-EPMC4060695 | biostudies-other | 2014 Jun

REPOSITORIES: biostudies-other

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