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Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction.


ABSTRACT: Controlled mechanical ventilation (CMV) is associated with the development of diaphragm atrophy and contractile dysfunction, and respiratory muscle weakness is thought to contribute significantly to delayed weaning of patients. Therefore, therapeutic strategies for preventing these processes may have clinical benefit. The aim of the current study was to investigate the role of the Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) signaling pathway in CMV-mediated diaphragm wasting and weakness in rats. CMV-induced diaphragm atrophy and contractile dysfunction coincided with marked increases in STAT3 phosphorylation on both tyrosine 705 (Tyr705) and serine 727 (Ser727). STAT3 activation was accompanied by its translocation into mitochondria within diaphragm muscle and mitochondrial dysfunction. Inhibition of JAK signaling during CMV prevented phosphorylation of both target sites on STAT3, eliminated the accumulation of phosphorylated STAT3 within the mitochondria, and reversed the pathologic alterations in mitochondrial function, reduced oxidative stress in the diaphragm, and maintained normal diaphragm contractility. In addition, JAK inhibition during CMV blunted the activation of key proteolytic pathways in the diaphragm, as well as diaphragm atrophy. These findings implicate JAK/STAT3 signaling in the development of diaphragm muscle atrophy and dysfunction during CMV and suggest that the delayed extubation times associated with CMV can be prevented by inhibition of Janus kinase signaling.-Smith, I. J., Godinez, G. L., Singh, B. K., McCaughey, K. M., Alcantara, R. R., Gururaja, T., Ho, M. S., Nguyen, H. N., Friera, A. M., White, K. A., McLaughlin, J. R., Hansen, D., Romero, J. M., Baltgalvis, K. A., Claypool, M. D., Li, W., Lang, W., Yam, G. C., Gelman, M. S., Ding, R., Yung, S. L., Creger, D. P., Chen, Y., Singh, R., Smuder, A. J., Wiggs, M. P., Kwon, O.-S., Sollanek, K. J., Powers, S. K., Masuda, E. S., Taylor, V. C., Payan, D. G., Kinoshita, T., Kinsella, T. M. Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction.

SUBMITTER: Smith IJ 

PROVIDER: S-EPMC4062832 | biostudies-other | 2014 Jul

REPOSITORIES: biostudies-other

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Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction.

Smith Ira J IJ   Godinez Guillermo L GL   Singh Baljit K BK   McCaughey Kelly M KM   Alcantara Raniel R RR   Gururaja Tarikere T   Ho Melissa S MS   Nguyen Henry N HN   Friera Annabelle M AM   White Kathy A KA   McLaughlin John R JR   Hansen Derek D   Romero Jason M JM   Baltgalvis Kristen A KA   Claypool Mark D MD   Li Wei W   Lang Wayne W   Yam George C GC   Gelman Marina S MS   Ding Rongxian R   Yung Stephanie L SL   Creger Daniel P DP   Chen Yan Y   Singh Rajinder R   Smuder Ashley J AJ   Wiggs Michael P MP   Kwon Oh-Sung OS   Sollanek Kurt J KJ   Powers Scott K SK   Masuda Esteban S ES   Taylor Vanessa C VC   Payan Donald G DG   Kinoshita Taisei T   Kinsella Todd M TM  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20140326 7


Controlled mechanical ventilation (CMV) is associated with the development of diaphragm atrophy and contractile dysfunction, and respiratory muscle weakness is thought to contribute significantly to delayed weaning of patients. Therefore, therapeutic strategies for preventing these processes may have clinical benefit. The aim of the current study was to investigate the role of the Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) signaling pathway in CMV-mediated diap  ...[more]

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