Unknown

Dataset Information

0

Cellular resolution functional imaging in behaving rats using voluntary head restraint.


ABSTRACT: High-throughput operant conditioning systems for rodents provide efficient training on sophisticated behavioral tasks. Combining these systems with technologies for cellular resolution functional imaging would provide a powerful approach to study neural dynamics during behavior. Here we describe an integrated two-photon microscope and behavioral apparatus that allows cellular resolution functional imaging of cortical regions during epochs of voluntary head restraint. Rats were trained to initiate periods of restraint up to 8 s in duration, which provided the mechanical stability necessary for in vivo imaging while allowing free movement between behavioral trials. A mechanical registration system repositioned the head to within a few microns, allowing the same neuronal populations to be imaged on each trial. In proof-of-principle experiments, calcium-dependent fluorescence transients were recorded from GCaMP-labeled cortical neurons. In contrast to previous methods for head restraint, this system can be incorporated into high-throughput operant conditioning systems.

SUBMITTER: Scott BB 

PROVIDER: S-EPMC4068252 | biostudies-other | 2013 Oct

REPOSITORIES: biostudies-other

altmetric image

Publications

Cellular resolution functional imaging in behaving rats using voluntary head restraint.

Scott Benjamin B BB   Brody Carlos D CD   Tank David W DW  

Neuron 20130919 2


High-throughput operant conditioning systems for rodents provide efficient training on sophisticated behavioral tasks. Combining these systems with technologies for cellular resolution functional imaging would provide a powerful approach to study neural dynamics during behavior. Here we describe an integrated two-photon microscope and behavioral apparatus that allows cellular resolution functional imaging of cortical regions during epochs of voluntary head restraint. Rats were trained to initiat  ...[more]

Similar Datasets

| S-EPMC2872549 | biostudies-literature
| S-EPMC4776509 | biostudies-literature
| S-EPMC10227058 | biostudies-literature
| S-EPMC4857106 | biostudies-literature
| S-EPMC6872567 | biostudies-literature
| S-EPMC4853475 | biostudies-literature
| S-EPMC8236375 | biostudies-literature
| S-EPMC8034419 | biostudies-literature
| S-EPMC9795332 | biostudies-literature
| S-EPMC2967725 | biostudies-literature