Ontology highlight
ABSTRACT:
SUBMITTER: Choi NY
PROVIDER: S-EPMC4086341 | biostudies-other | 2014 Jun
REPOSITORIES: biostudies-other
Choi Na Young NY Park Yo Seph YS Ryu Jae-Sung JS Lee Hye Jeong HJ Araúzo-Bravo Marcos J MJ Ko Kisung K Han Dong Wook DW Schöler Hans R HR Ko Kinarm K
Molecules and cells 20140523 6
Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free c ...[more]