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Plant members of a family of sulfate transporters reveal functional subtypes.

ABSTRACT: Three plant sulfate transporter cDNAs have been isolated by complementation of a yeast mutant with a cDNA library derived from the tropical forage legume Stylosanthes hamata. Two of these cDNAs, shst1 and shst2, encode high-affinity H+/sulfate cotransporters that mediate the uptake of sulfate by plant roots from low concentrations of sulfate in the soil solution. The third, shst3, represents a different subtype encoding a lower affinity H+/sulfate cotransporter, which may be involved in the internal transport of sulfate between cellular or subcellular compartments within the plant. The steady-state level of mRNA corresponding to both subtypes is subject to regulation by signals that ultimately respond to the external sulfate supply. These cDNAs represent the identification of plant members of a family of related sulfate transporter proteins whose sequences exhibit significant amino acid conservation in filamentous fungi, yeast, plants, and mammals.

SUBMITTER: Smith FW

PROVIDER: S-EPMC40987 | biostudies-other | 1995 Sep

REPOSITORIES: biostudies-other

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Plant members of a family of sulfate transporters reveal functional subtypes.

Smith F W FW Ealing P M PM Hawkesford M J MJ Clarkson D T DT

Proceedings of the National Academy of Sciences of the United States of America 19950901 20

Three plant sulfate transporter cDNAs have been isolated by complementation of a yeast mutant with a cDNA library derived from the tropical forage legume Stylosanthes hamata. Two of these cDNAs, shst1 and shst2, encode high-affinity H+/sulfate cotransporters that mediate the uptake of sulfate by plant roots from low concentrations of sulfate in the soil solution. The third, shst3, represents a different subtype encoding a lower affinity H+/sulfate cotransporter, which may be involved in the inte ...[more]

PMID: 7568135

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Dataset Information

Plant members of a family of sulfate transporters reveal functional subtypes.

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Plant members of a family of sulfate transporters reveal functional subtypes.

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