Ontology highlight
ABSTRACT:
SUBMITTER: Poon D
PROVIDER: S-EPMC41129 | biostudies-other | 1995 Aug
REPOSITORIES: biostudies-other
Poon D D Bai Y Y Campbell A M AM Bjorklund S S Kim Y J YJ Zhou S S Kornberg R D RD Weil P A PA
Proceedings of the National Academy of Sciences of the United States of America 19950801 18
Although the mechanisms of transcriptional regulation by RNA polymerase II are apparently highly conserved from yeast to man, the identification of a yeast TATA-binding protein (TBP)-TBP-associated factor (TAFII) complex comparable to the metazoan TFIID component of the basal transcriptional machinery has remained elusive. Here, we report the isolation of a yeast TBP-TAFII complex which can mediate transcriptional activation by GAL4-VP16 in a highly purified yeast in vitro transcription system. ...[more]