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An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.

ABSTRACT: We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles.

SUBMITTER: Stamnes MA

PROVIDER: S-EPMC41276 | biostudies-other | 1995 Aug

REPOSITORIES: biostudies-other

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An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.

Stamnes M A MA Craighead M W MW Hoe M H MH Lampen N N Geromanos S S Tempst P P Rothman J E JE

Proceedings of the National Academy of Sciences of the United States of America 19950801 17

We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesic ...[more]

PMID: 7644530

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A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding.

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