Ontology highlight
ABSTRACT:
SUBMITTER: Lee SI
PROVIDER: S-EPMC41563 | biostudies-other | 1995 Jul
REPOSITORIES: biostudies-other
Lee S I SI Umen J G JG Varmus H E HE
Proceedings of the National Academy of Sciences of the United States of America 19950701 14
To identify cellular factors that function in -1 ribosomal frameshifting, we have developed assays in the yeast Saccharomyces cerevisiae to screen for host mutants in which frameshifting is specifically affected. Expression vectors have been constructed in which the mouse mammary tumor virus gag-pro frameshift region is placed upstream of the lacZ gene or the CUP1 gene so that the reporters are in the -1 frame relative to the initiation codon. These vectors have been used to demonstrate that -1 ...[more]