Ontology highlight
ABSTRACT:
SUBMITTER: Myers WK
PROVIDER: S-EPMC4156861 | biostudies-other | 2014 Sep
REPOSITORIES: biostudies-other
Myers William K WK Stich Troy A TA Suess Daniel L M DL Kuchenreuther Jon M JM Swartz James R JR Britt R David RD
Journal of the American Chemical Society 20140825 35
The two cyanide ligands in the assembled cluster of [FeFe] hydrogenase originate from exogenous l-tyrosine. Using selectively labeled tyrosine substrates, the cyanides were isotopically labeled via a recently developed in vitro maturation procedure allowing advanced electron paramagnetic resonance techniques to probe the electronic structure of the catalytic core of the enzyme. The ratio of the isotropic (13)C hyperfine interactions for the two CN(-) ligands-a reporter of spin density on their r ...[more]