In vitro nanobody discovery for integral membrane protein targets.
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ABSTRACT: Nanobodies (Nbs) or single-domain antibodies are among the smallest and most stable binder scaffolds known. In vitro display is a powerful antibody discovery technique used worldwide. We describe the first adaptation of in vitro mRNA/cDNA display for the rapid, automatable discovery of Nbs against desired targets, and use it to discover the first ever reported nanobody against the human full-length glucose transporter, GLUT-1. We envision our streamlined method as a bench-top platform technology, in combination with various molecular evolution techniques, for expedited Nb discovery.
SUBMITTER: Doshi R
PROVIDER: S-EPMC4208029 | biostudies-other | 2014 Oct
REPOSITORIES: biostudies-other
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