Ontology highlight
ABSTRACT:
SUBMITTER: Salmela L
PROVIDER: S-EPMC4253826 | biostudies-other | 2014 Dec
REPOSITORIES: biostudies-other
Bioinformatics (Oxford, England) 20140826 24
<h4>Motivation</h4>PacBio single molecule real-time sequencing is a third-generation sequencing technique producing long reads, with comparatively lower throughput and higher error rate. Errors include numerous indels and complicate downstream analysis like mapping or de novo assembly. A hybrid strategy that takes advantage of the high accuracy of second-generation short reads has been proposed for correcting long reads. Mapping of short reads on long reads provides sufficient coverage to elimin ...[more]