Ontology highlight
ABSTRACT:
SUBMITTER: Irion U
PROVIDER: S-EPMC4299274 | biostudies-other | 2014 Dec
REPOSITORIES: biostudies-other
Irion Uwe U Krauss Jana J Nüsslein-Volhard Christiane C
Development (Cambridge, England) 20141119 24
The introduction of engineered site-specific DNA endonucleases has brought precise genome editing in many model organisms and human cells into the realm of possibility. In zebrafish, loss-of-function alleles have been successfully produced; however, germ line transmission of functional targeted knock-ins of protein tags or of SNP exchanges have not been reported. Here we show by phenotypic rescue that the CRISPR/Cas system can be used to target and repair a premature stop codon at the albino (al ...[more]