Microcontroller-assisted compensation of adenosine triphosphate levels: instrument and method development.
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ABSTRACT:
SUBMITTER: Hu JB
PROVIDER: S-EPMC4311256 | biostudies-other | 2015
REPOSITORIES: biostudies-other
Publications
Microcontroller-assisted compensation of adenosine triphosphate levels: instrument and method development.
Hu Jie-Bi JB Chen Ting-Ru TR Chen Yu-Chie YC Urban Pawel L PL
Scientific reports 20150130
In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme. ...[more]
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