Ontology highlight
ABSTRACT:
SUBMITTER: Hwang L
PROVIDER: S-EPMC4372338 | biostudies-other | 2015 Feb
REPOSITORIES: biostudies-other
Hwang Leekyoung L Ayaz-Guner Serife S Gregorich Zachery R ZR Cai Wenxuan W Valeja Santosh G SG Jin Song S Ge Ying Y
Journal of the American Chemical Society 20150211 7
Analysis of protein phosphorylation remains a significant challenge due to the low abundance of phosphoproteins and the low stoichiometry of phosphorylation, which requires effective enrichment of phosphoproteins. Here we have developed superparamagnetic nanoparticles (NPs) whose surface is functionalized by multivalent ligand molecules that specifically bind to the phosphate groups on any phosphoproteins. These NPs enrich phosphoproteins from complex cell and tissue lysates with high specificit ...[more]