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Structure and cell cycle-regulated transcription of the human cyclin A gene.

ABSTRACT: Cyclin A is a cell cycle regulatory protein that functions in mitotic and S-phase control in mammalian somatic cells. Its deregulated expression may have a role in cellular transformation. We have cloned and sequenced the human cyclin A gene and cDNAs representing its mRNAs and have characterized its promoter. Using synchronized cultures of NIH 3T3 cells stably transfected with cyclin A promoter/luciferase constructs, we show that the promoter is repressed during the G1 phase of the cell cycle and is activated at S-phase entry. Cell cycle regulation of the cyclin A gene promoter is mediated by sequences extending from -79 to +100 relative to the predominant transcription start site. It does not require the presence of a functional retinoblastoma protein.

SUBMITTER: Henglein B

PROVIDER: S-EPMC44021 | biostudies-other | 1994 Jun

REPOSITORIES: biostudies-other

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Structure and cell cycle-regulated transcription of the human cyclin A gene.

Henglein B B Chenivesse X X Wang J J Eick D D Bréchot C C

Proceedings of the National Academy of Sciences of the United States of America 19940601 12

Cyclin A is a cell cycle regulatory protein that functions in mitotic and S-phase control in mammalian somatic cells. Its deregulated expression may have a role in cellular transformation. We have cloned and sequenced the human cyclin A gene and cDNAs representing its mRNAs and have characterized its promoter. Using synchronized cultures of NIH 3T3 cells stably transfected with cyclin A promoter/luciferase constructs, we show that the promoter is repressed during the G1 phase of the cell cycle a ...[more]

PMID: 8202514

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