Ontology highlight
ABSTRACT:
SUBMITTER: Fischer R
PROVIDER: S-EPMC4409837 | biostudies-other | 2015 Apr
REPOSITORIES: biostudies-other
Proteomics 20150205 7
We describe a "gel-assisted" proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sa ...[more]