Ontology highlight
ABSTRACT:
SUBMITTER: Escuin S
PROVIDER: S-EPMC4510849 | biostudies-other | 2015 Jul
REPOSITORIES: biostudies-other
Escuin Sarah S Vernay Bertrand B Savery Dawn D Gurniak Christine B CB Witke Walter W Greene Nicholas D E ND Copp Andrew J AJ
Journal of cell science 20150603 14
The cytoskeleton is widely considered essential for neurulation, yet the mouse spinal neural tube can close despite genetic and non-genetic disruption of the cytoskeleton. To investigate this apparent contradiction, we applied cytoskeletal inhibitors to mouse embryos in culture. Preventing actomyosin cross-linking, F-actin assembly or myosin II contractile activity did not disrupt spinal closure. In contrast, inhibiting Rho kinase (ROCK, for which there are two isoforms ROCK1 and ROCK2) or block ...[more]