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A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases.

ABSTRACT: A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

SUBMITTER: Angelini LM 

PROVIDER: S-EPMC4512080 | biostudies-other | 2015 Mar

REPOSITORIES: biostudies-other

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A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases.

Angelini Leticia Mara Lima LM   da Silva Amanda Ribeiro Martins AR   Rocco Lucas de Freitas Coli Lde F   Milagre Cintia Duarte de Freitas CD  

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20150331 1

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects. ...[more]