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Glucocorticoid affects dendritic transport of BDNF-containing vesicles.


ABSTRACT: Brain-derived neurotrophic factor (BDNF) is essential for neuronal survival, differentiation, and functions in the central nervous system (CNS). Because BDNF protein is sorted into secretory vesicles at the trans-Golgi network in the cell body after translation, transport of BDNF-containing vesicles to the secretion sites is an important process for its function. Here we examined the effect of dexamethasone (DEX), a synthetic glucocorticoid, on BDNF-containing vesicle transport and found that DEX decreased the proportion of stationary vesicles and increased velocity of the microtubule-based vesicle transport in dendrites of cortical neurons. Furthermore, DEX increased huntingtin (Htt) protein levels via glucocorticoid receptor (GR) activation, and reduction in the amount of Htt by a specific shRNA reversed the action of DEX on BDNF vesicle transport. Given that Htt protein is a positive regulator for the microtubule-dependent vesicular transport in neurons, our data suggest that glucocorticoid stimulates BDNF vesicle transport through upregulation of Htt protein levels.

SUBMITTER: Adachi N 

PROVIDER: S-EPMC4523857 | biostudies-other | 2015 Aug

REPOSITORIES: biostudies-other

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Glucocorticoid affects dendritic transport of BDNF-containing vesicles.

Adachi Naoki N   Numakawa Tadahiro T   Nakajima Shingo S   Fukuoka Masashi M   Odaka Haruki H   Katanuma Yusuke Y   Ooshima Yoshiko Y   Hohjoh Hirohiko H   Kunugi Hiroshi H  

Scientific reports 20150804


Brain-derived neurotrophic factor (BDNF) is essential for neuronal survival, differentiation, and functions in the central nervous system (CNS). Because BDNF protein is sorted into secretory vesicles at the trans-Golgi network in the cell body after translation, transport of BDNF-containing vesicles to the secretion sites is an important process for its function. Here we examined the effect of dexamethasone (DEX), a synthetic glucocorticoid, on BDNF-containing vesicle transport and found that DE  ...[more]

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