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Crystal structure of intercalated four-stranded d(C3T) at 1.4 A resolution.

ABSTRACT: The crystal structure of d(C3T), solved at 1.4 A resolution, reveals that the molecule forms a four-stranded intercalated complex. It consists of two parallel-stranded duplexes, each of which is held together by cytosine-protonated cytosine base pairs. The two duplexes are intercalated with each other and have opposite strand orientation. The molecule has a flat, lath-like appearance, and the covalently bonded cytosines have a slow right-handed twist of 17.1 degrees. However, there is considerable asymmetry. On one of the flat sides, the phosphate groups are rotated away from the center of the molecule. They are held in this orientation by bridging water molecules that bind the NH of cytosine and a phosphate group of an opposite chain. There is also considerable microheterogeneity in the structure. The cytosine hemiprotonation occurs even at pH 7 where stable crystals form.

SUBMITTER: Kang CH

PROVIDER: S-EPMC45286 | biostudies-other | 1994 Nov

REPOSITORIES: biostudies-other

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Crystal structure of intercalated four-stranded d(C3T) at 1.4 A resolution.

Kang C H CH Berger I I Lockshin C C Ratliff R R Moyzis R R Rich A A

Proceedings of the National Academy of Sciences of the United States of America 19941101 24

The crystal structure of d(C3T), solved at 1.4 A resolution, reveals that the molecule forms a four-stranded intercalated complex. It consists of two parallel-stranded duplexes, each of which is held together by cytosine-protonated cytosine base pairs. The two duplexes are intercalated with each other and have opposite strand orientation. The molecule has a flat, lath-like appearance, and the covalently bonded cytosines have a slow right-handed twist of 17.1 degrees. However, there is considerab ...[more]

PMID: 7972115

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