In vitro detection of circulating tumor cells compared by the CytoTrack and CellSearch methods.

ABSTRACT: Comparison of two methods to detect circulating tumor cells (CTC) CytoTrack and CellSearch through recovery of MCF-7 breast cancer cells, spiked into blood collected from healthy donors. Spiking of a fixed number of EpCAM and pan-cytokeratin positive MCF-7 cells into 7.5 mL donor blood was performed by FACSAria flow sorting. The samples were shipped to either CytoTrack or CellSearch research facilities within 48 h, where evaluation of MCF-7 recovery was performed. CytoTrack and CellSearch analyses were performed simultaneously. Recoveries of MCF-7 single cells, cells in clusters, and clusters were determined. The average numbers of MCF-7 cells/cells in clusters/clusters recovered from blood by the CytoTrack and CellSearch methods were 103?±?5.9/27?±?7.9/11?±?3.5 (95 % CI) and 107?±?4.4/20?±?7.1/10?±?3.5, respectively, with no difference between the two methods (p?=?0.37/p?=?0.23/p?=?0.09). Overall, the recovery of CytoTrack and CellSearch was 68.8?±?3.9 %/71.1?±?2.9 %, respectively (p?=?0.58). In spite of different methodologies, CytoTrack and CellSearch found similar number of CTCs, when spiking was performed with the EpCAM and pan cytokeratin-positive cell line MCF-7. The results suggest that CytoTrack and CellSearch have similar abilities to identify CTC in vitro.

SUBMITTER: Hillig T 

PROVIDER: S-EPMC4529454 | biostudies-other | 2015 Jun

REPOSITORIES: biostudies-other