Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Plasmonic Gold Decorated MWCNT Nanocomposite for Localized Plasmon Resonance Sensing.

ABSTRACT: The synergism of excellent properties of carbon nanotubes and gold nanoparticles is used in this work for bio-sensing of recombinant bovine growth hormones (rbST) by making Multi Wall Carbon Nanotubes (MWCNT) locally optically responsive by augmenting it optical properties through Localized Surface Plasmon Resonance (LSPR). To this purpose, locally gold nano particles decorated gold-MWCNT composite was synthesized from a suspension of MWCNT bundles and hydrogen chloroauric acid in an aqueous solution, activated ultrasonically and, then, drop-casted on a glass substrate. The slow drying of the drop produces a "coffee ring" pattern that is found to contain gold-MWCNT nanocomposites, accumulated mostly along the perimeter of the ring. The reaction is studied also at low-temperature, in the vacuum chamber of the Scanning Electron Microscope and is accounted for by the local melting processes that facilitate the contact between the bundle of tubes and the gold ions. Biosensing applications of the gold-MWCNT nanocomposite using their LSPR properties are demonstrated for the plasmonic detection of traces of bovine growth hormone. The sensitivity of the hybrid platform which is found to be 1 ng/ml is much better than that measuring with gold nanoparticles alone which is only 25 ng/ml.

SUBMITTER: Ozhikandathil J

PROVIDER: S-EPMC4539546 | biostudies-other | 2015

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Au-WO3 Nanocomposite Coatings for Localized Surface Plasmon Resonance Sensing.

Project description:Localized surface plasmon resonance (LSPR) gas sensors are gaining increasing importance due to their unique tuneable functional properties. Au-WO3-x nanocomposite coatings, in particular, can be outstandingly sensitive to many different gases. However, a proper understanding of their optical properties and the way in which those properties are correlated to their structure/microstructure, is still needed. In this work, Au-WO3 nanocomposite coatings, with Au contents between 0-11 atomic percent, were grown using reactive magnetron co-sputtering technique and were characterized concerning their optical response. The precipitation of Au nanoparticles in the oxide matrix was promoted through thermal annealing treatments until 500 °C. Along with the Au nanoparticles' morphological changes, the annealing treatments stimulated the crystallization of WO3, together with the appearance of oxygen-deficient WO3-x phases. Through theoretical simulations, we have related the LSPR effect with the different structural and morphological variations (namely, size and distribution of the nanoparticles and their local environment), which were a function of the Au content and annealing temperature. Our results suggest that local voids were present in the vicinity of the Au nanoparticles, for all temperature range, and that they should be present in a wide variety of Au-WO3 nanocomposites. A theoretical study concerning the refractive index sensitivity was carried out in order to predict the optimal coating design parameters for gas sensing experiments.

| S-EPMC6981964 | biostudies-literature

Multispectral Localized Surface Plasmon Resonance (msLSPR) Reveals and Overcomes Spectral and Sensing Heterogeneities of Single Gold Nanoparticles.

Project description:Metal nanoparticles can be sensitive molecular sensors due to enhanced absorption and scattering of light near a localized surface plasmon resonance (LSPR). Variations in both intrinsic properties such as the geometry and extrinsic properties such as the environment can cause heterogeneity in nanoparticle LSPR and impact the overall sensing responses. To date, however, few studies have examined LSPR and sensing heterogeneities, due to technical challenges in obtaining the full LSPR spectra of individual nanoparticles in dynamic assays. Here, we report multispectral LSPR (msLSPR), a wide-field imaging technique for real-time spectral monitoring of light scattering from individual nanoparticles across the whole field of view (FOV) at ∼0.5 nm spectral and ∼100 ms temporal resolutions. Using msLSPR, we studied the spectral and sensing properties of gold nanoparticles commonly used in LSPR assays, including spheres, rods, and bipyramids. Complemented with electron microscopy imaging, msLSPR analysis revealed that all classes of gold nanoparticles exhibited variations in LSPR peak wavelengths that largely paralleled variations in morphology. Compared with the rods and spheres, gold nanobipyramids exhibited both more uniform and stronger sensing responses as long as the bipyramids are structurally intact. Simulations incorporating the experimental LSPR properties demonstrate the negative impact of spectral heterogeneity on the overall performance of conventional, intensity-based LSPR assays and the ability of msLSPR in overcoming both particle heterogeneity and measurement noise. These results highlight the importance of spectral heterogeneity in LSPR-based sensors and the potential advantage of performing LSPR assays in the spectral domain.

| S-EPMC9933608 | biostudies-literature

Gas sensing with high-resolution localized surface plasmon resonance spectroscopy.

Project description:We report the first inert gas sensing and characterization studies based on high-resolution localized surface plasmon resonance (HR-LSPR) spectroscopy. HR-LSPR was used to detect the extremely small changes (<3 × 10(-4)) in bulk refractive index when the gas was switched between He(g) and Ar(g) or He(g) and N2(g). We also demonstrate submonolayer sensitivity to adsorbed water from exposure of the sensor to air (40% humidity) versus dry N2(g). These measurements significantly expand the applications space and characterization tools for plasmonic nanosensors.

| S-EPMC3084350 | biostudies-literature

Localized surface plasmon resonance-based abscisic acid biosensor using aptamer-functionalized gold nanoparticles.

Project description:Abscisic acid (ABA) plays an important role in abiotic stress response and physiological signal transduction resisting to the adverse environment. Therefore, it is very essential for the quantitative detection of abscisic acid (ABA) due to its indispensable role in plant physiological activities. Herein, a new detection method based on localized surface plasmon resonance (LSPR) using aptamer-functionalized gold nanoparticles (AuNPs) is developed without using expensive instrument and antibody. In the presence of ABA, ABA specifically bind with their aptamers to form the ABA-aptamer complexes with G-quadruplex-like structure and lose the ability to stabilize AuNPs against NaCl-induced aggregation. Meanwhile, the changes of the LSPR spectra of AuNP solution occur and therefore the detection of ABA achieved. Under optimized conditions, this method showed a good linear range covering from 5×10-7 M to 5×10-5 M with a detection limit of 0.33 μM. In practice, the usage of this novel method has been demonstrated by its application to detect ABA from fresh leaves of rice with the relative error of 6.59%-7.93% compared with ELISA bioassay. The experimental results confirmed that this LSPR-based biosensor is simple, selective and sensitive for the detection of ABA. The proposed LSPR method could offer a new analytical platform for the detection of other plant hormones by changing the corresponding aptamer.

| S-EPMC5617216 | biostudies-literature

Brightening gold nanoparticles: new sensing approach based on plasmon resonance energy transfer.

Project description:Scattering recovered plasmonic resonance energy transfer (SR-PRET) was reported by blocking the plasmon resonance energy transfer (PRET) from gold nanoparticle (GNP) to the adsorbed molecules (RdBS). Due to the selective cleavage of the Si-O bond by F- ions, the quenching is switched off causing an increase in the brightness of the GNPs,detected using dark-field microscopy (DFM) were brightened. This method was successfully applied to the determination of fluoride ions in water. The SR-PRET provides a potential approach for a vitro/vivo sensing with high sensitivity and selectivity.

| S-EPMC4650782 | biostudies-literature

An innovative application of time-domain spectroscopy on localized surface plasmon resonance sensing.

Project description:White-light scanning interferometry (WLSI) is often used to study the surface profiles and properties of thin films because the strength of the technique lies in its ability to provide fast and high resolution measurements. An innovative attempt is made in this paper to apply WLSI as a time-domain spectroscopic system for localized surface plasmon resonance (LSPR) sensing. A WLSI-based spectrometer is constructed with a breadboard of WLSI in combination with a spectral centroid algorithm for noise reduction and performance improvement. Experimentally, the WLSI-based spectrometer exhibits a limit of detection (LOD) of 1.2 × 10-3 refractive index units (RIU), which is better than that obtained with a conventional UV-Vis spectrometer, by resolving the LSPR peak shift. Finally, the bio-applicability of the proposed spectrometer was investigated using the rs242557 tau gene, an Alzheimer's and Parkinson's disease biomarker. The LOD was calculated as 15 pM. These results demonstrate that the proposed WLSI-based spectrometer could become a sensitive time-domain spectroscopic biosensing platform.

| S-EPMC5345092 | biostudies-literature

Au nano-urchins enabled localized surface plasmon resonance sensing of beta amyloid fibrillation.

Project description:Early stage detection of neurodegenerative diseases such as Alzheimer's disease (AD) is of utmost importance, as it has become one of the leading causes of death of millions of people. The gradual intellectual decline in AD patients is an outcome of fibrillation of amyloid beta 1-42 (Aβ1-42) peptides in the brain. In this paper, we present localized surface plasmon resonance (LSPR) based sensing of Aβ1-42 fibrillation using Au nano-urchins. Strongly localized field confinement at the spiky nanostructures of nano-urchin surfaces enables them to detect very low concentrations of Aβ1-42. In addition, the LSPR peak of Au nano-urchins, which is very sensitive to ambient conditions, shows significant responses at different fibrillation stages of Aβ1-42. Reduction in LSPR peak intensity with an increase in the fibrillation is chosen as the sensing parameter here. This paper in this context provides LSPR based highly sensitive, label-free and real-time sensing of Aβ1-42 fibrillation that is highly advantageous compared to the existing techniques which require binding additives or fluorescent biomarkers.

| S-EPMC9417577 | biostudies-literature

Investigation of Performance Parameters of Spherical Gold Nanoparticles in Localized Surface Plasmon Resonance Biosensing.

Project description:In this paper, we present numerical and experimental results on Localized Surface Plasmon Resonance (LSPR) refractive index (RI) sensitivity, Figure of Merit (FoM), and penetration depth (dp) dependence on spherical gold nanoparticles (AuNPs) size, and the effects of AuNP dimer interparticle distance (ds) studied numerically. These parameters were calculated and observed for d = 20, 40, 60, 80, and 100 nm diameter spherical AuNPs. Our investigation shows d = 60 nm AuNPs give the best FoM. The AuNP dimer interparticle distance can significantly influence the RI sensitivity. Therefore, the effect of distances between pairs of d = 20 nm and 60 nm AuNPs is shown. We discuss the importance of penetration depth information for AuNPs functionalized with aptamers for biosensing in the context of aptamer size.

| S-EPMC10535430 | biostudies-literature

Localized surface plasmon resonance shift of biosynthesized and functionalized quasi-spherical gold nanoparticle systems.

Project description:Rapid and more environment-friendly means of gold nanoparticle synthesis is necessary in many applications, as in ion detection. Leaf extracts have become effective and economical reducing agents for gold nanoparticle formation, however, effects of extract combinations have not been thoroughly investigated. With the exploitation of combined extract effects, gold nanoparticles were synthesized then functionalized and investigated to produce selected nanoparticle systems which are capable of detecting aqueous lead(ii) ions with minimum detection limits of 10-11 ppm. The measured localized surface plasmon resonance absorption peaks of the gold nanoparticles were 541-800 nm for the synthesis and 549 nm for the functionalization. The diameters of different gold nanoparticle systems were 17-37 nm. These were mostly quasi-spherical in morphology with some rod-, triangular-, and hexagonal plate-like particles. The biosynthesis used polyphenols and acids present in the extracts in the reduction of gold ions into gold nanoparticles, and in the nanoparticle capping and stabilization. Functionalization replaced the capping compounds with alliin, S-allylcysteine, allicin, and ajoene. Gold nanoparticle stability in aqueous systems was verified for two weeks up to five months. The investigations concluded the practicability of the gold nanoparticles in lead(ii) ion detection with selectivity initially verified for other divalent cations.

| S-EPMC10424193 | biostudies-literature

Profile prediction and fabrication of wet-etched gold nanostructures for localized surface plasmon resonance.

Project description:Dispersed nanosphere lithography can be employed to fabricate gold nanostructures for localized surface plasmon resonance, in which the gold film evaporated on the nanospheres is anisotropically dry etched to obtain gold nanostructures. This paper reports that by wet etching of the gold film, various kinds of gold nanostructures can be fabricated in a cost-effective way. The shape of the nanostructures is predicted by profile simulation, and the localized surface plasmon resonance spectrum is observed to be shifting its extinction peak with the etching time.(See supplementary material 1).

| S-EPMC2894108 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data