Ontology highlight
ABSTRACT:
SUBMITTER: Angus SV
PROVIDER: S-EPMC4549193 | biostudies-other | 2015 Dec
REPOSITORIES: biostudies-other
Angus Scott V SV Cho Soohee S Harshman Dustin K DK Song Jae-Young JY Yoon Jeong-Yeol JY
Biosensors & bioelectronics 20150629
A novel polymerase chain reaction (PCR) device was developed that uses wire-guided droplet manipulation (WDM) to guide a droplet over three different heating chambers. After PCR amplification, end-point detection is achieved using a smartphone-based fluorescence microscope. The device was tested for identification of the 16S rRNA gene V3 hypervariable region from Escherichia coli genomic DNA. The lower limit of detection was 10(3) genome copies per sample. The device is portable with smartphone- ...[more]