Unknown

Dataset Information

0

Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.


ABSTRACT: Thrombopoietin (TPO), a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Recently we have isolated a cDNA encoding a ligand for the murine c-mpl protooncogene and shown it to be TPO. By employing a murine cDNA probe, we have isolated a gene encoding human TPO from a human genomic library. The TPO locus spans over 6 kb and has a structure similar to that of the erythropoietin gene (EPO). Southern blot analysis of human genomic DNA reveals a hybridization pattern consistent with a single gene locus. The locus was mapped by in situ hybridization of metaphase chromosome preparations to chromosome 3q26-27, a site where a number of chromosomal abnormalities associated with thrombocythemia in cases of acute myeloid leukemia have been mapped. A human TPO cDNA was isolated by PCR from kidney mRNA. The cDNA encodes a protein with 80% identity to previously described murine TPO and is capable of initiating a proliferative signal to murine interleukin 3-dependent BaF3 cells expressing the murine or human TPO receptor.

SUBMITTER: Foster DC 

PROVIDER: S-EPMC45573 | biostudies-other | 1994 Dec

REPOSITORIES: biostudies-other

altmetric image

Publications

Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.

Foster D C DC   Sprecher C A CA   Grant F J FJ   Kramer J M JM   Kuijper J L JL   Holly R D RD   Whitmore T E TE   Heipel M D MD   Bell L A LA   Ching A F AF  

Proceedings of the National Academy of Sciences of the United States of America 19941201 26


Thrombopoietin (TPO), a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Recently we have isolated a cDNA encoding a ligand for the murine c-mpl protooncogene and shown it to be TPO. By employing a murine cDNA probe, we have isolated a gene encoding human TPO from a human genomic library. The TPO locus spans over 6 kb and has a structure simil  ...[more]

Similar Datasets

| S-EPMC308480 | biostudies-other
| S-EPMC50872 | biostudies-other
| S-EPMC1136538 | biostudies-other
| S-EPMC1219593 | biostudies-other
| S-EPMC122089 | biostudies-literature
| S-EPMC22698 | biostudies-literature
| S-EPMC1217674 | biostudies-other
| S-EPMC1222512 | biostudies-other
| S-EPMC4445085 | biostudies-literature
| S-EPMC42334 | biostudies-other