Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Camera array based light field microscopy.

ABSTRACT: This paper proposes a novel approach for high-resolution light field microscopy imaging by using a camera array. In this approach, we apply a two-stage relay system for expanding the aperture plane of the microscope into the size of an imaging lens array, and utilize a sensor array for acquiring different sub-apertures images formed by corresponding imaging lenses. By combining the rectified and synchronized images from 5 × 5 viewpoints with our prototype system, we successfully recovered color light field videos for various fast-moving microscopic specimens with a spatial resolution of 0.79 megapixels at 30 frames per second, corresponding to an unprecedented data throughput of 562.5 MB/s for light field microscopy. We also demonstrated the use of the reported platform for different applications, including post-capture refocusing, phase reconstruction, 3D imaging, and optical metrology.

SUBMITTER: Lin X

PROVIDER: S-EPMC4574646 | biostudies-other | 2015 Sep

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Computational version of the correlation light-field camera.

Project description:Light-field cameras allow the acquisition of both the spatial and angular components of the light-field. The conventional way to perform such acquisitions leads to a strong spatio-angular resolution limitation but correlation-enabled plenoptic cameras have been introduced recently that relax this constraint. Here we use a computational version of this concept to acquire realistic light-fields images using a commercial DSLR Camera lens as an imaging system. By placing the image sensor in the focal plane of a lens, within the camera we ensure the acquisition of pure angular components together with the spatial information. We perform an acquisition presenting a high spatio-angular rays resolution obtained through a trade off of the temporal resolution. The acquisition reported is photo-realistic and the acquisition of diffraction limited features is observed with the setup. Finally, we demonstrate the refocusing abilities of the camera.

| S-EPMC9741593 | biostudies-literature

Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array.

Project description:Conventional light sheet fluorescence microscopy (LSFM) utilizes two perpendicularly arranged objective lenses for optical excitation and detection, respectively. Such a configuration often limits the use of high-numerical-aperture (NA) objectives or requires specially designed long-working-distance objectives. Here, a LSFM based on a micro-mirror array (MMA) to enable light sheet imaging with a single objective lens is reported. The planar fluorescent emission excited by the light sheet illumination is collected by the same objective, relayed onto an MMA and detected by a side-view camera. The proposed scheme makes LSFM compatible to single objective imaging system and shows promising candidacy for high spatiotemporal imaging.

| S-EPMC9648671 | biostudies-literature

Trilobite-inspired neural nanophotonic light-field camera with extreme depth-of-field.

Project description:A unique bifocal compound eye visual system found in the now extinct trilobite, Dalmanitina socialis, may enable them to be sensitive to the light-field information and simultaneously perceive both close and distant objects in the environment. Here, inspired by the optical structure of their eyes, we demonstrate a nanophotonic light-field camera incorporating a spin-multiplexed bifocal metalens array capable of capturing high-resolution light-field images over a record depth-of-field ranging from centimeter to kilometer scale, simultaneously enabling macro and telephoto modes in a snapshot imaging. By leveraging a multi-scale convolutional neural network-based reconstruction algorithm, optical aberrations induced by the metalens are eliminated, thereby significantly relaxing the design and performance limitations on metasurface optics. The elegant integration of nanophotonic technology with computational photography achieved here is expected to aid development of future high-performance imaging systems.

| S-EPMC9019092 | biostudies-literature

Full-field interferometric confocal microscopy using a VCSEL array.

Project description:We present an interferometric confocal microscope using an array of 1200 vertical cavity surface emitting lasers (VCSELs) coupled to a multimode fiber. Spatial coherence gating provides ~18,000 continuous virtual pinholes, allowing an entire en face plane to be imaged in a snapshot. This approach maintains the same optical sectioning as a scanning confocal microscope without moving parts, while the high power of the VCSEL array (?5??mW per laser) enables high-speed image acquisition with integration times as short as 100 ?s. Interferometric detection also recovers the phase of the image, enabling quantitative phase measurements and improving the contrast when imaging phase objects.

| S-EPMC4217487 | biostudies-literature

A modular hierarchical array camera.

Project description:Array cameras removed the optical limitations of a single camera and paved the way for high-performance imaging via the combination of micro-cameras and computation to fuse multiple aperture images. However, existing solutions use dense arrays of cameras that require laborious calibration and lack flexibility and practicality. Inspired by the cognition function principle of the human brain, we develop an unstructured array camera system that adopts a hierarchical modular design with multiscale hybrid cameras composing different modules. Intelligent computations are designed to collaboratively operate along both intra- and intermodule pathways. This system can adaptively allocate imagery resources to dramatically reduce the hardware cost and possesses unprecedented flexibility, robustness, and versatility. Large scenes of real-world data were acquired to perform human-centric studies for the assessment of human behaviours at the individual level and crowd behaviours at the population level requiring high-resolution long-term monitoring of dynamic wide-area scenes.

| S-EPMC7892845 | biostudies-literature

Integrated Array Tomography for 3D Correlative Light and Electron Microscopy.

Project description:Volume electron microscopy (EM) of biological systems has grown exponentially in recent years due to innovative large-scale imaging approaches. As a standalone imaging method, however, large-scale EM typically has two major limitations: slow rates of acquisition and the difficulty to provide targeted biological information. We developed a 3D image acquisition and reconstruction pipeline that overcomes both of these limitations by using a widefield fluorescence microscope integrated inside of a scanning electron microscope. The workflow consists of acquiring large field of view fluorescence microscopy (FM) images, which guide to regions of interest for successive EM (integrated correlative light and electron microscopy). High precision EM-FM overlay is achieved using cathodoluminescent markers. We conduct a proof-of-concept of our integrated workflow on immunolabelled serial sections of tissues. Acquisitions are limited to regions containing biological targets, expediting total acquisition times and reducing the burden of excess data by tens or hundreds of GBs.

| S-EPMC8809480 | biostudies-literature

Noise-robust phase-space deconvolution for light-field microscopy.

Project description:SignificanceLight-field microscopy has achieved success in various applications of life sciences that require high-speed volumetric imaging. However, existing light-field reconstruction algorithms degrade severely in low-light conditions, and the deconvolution process is time-consuming.AimThis study aims to develop a noise robustness phase-space deconvolution method with low computational costs.ApproachWe reformulate the light-field phase-space deconvolution model into the Fourier domain with random-subset ordering and total-variation (TV) regularization. Additionally, we build a time-division-based multicolor light-field microscopy and conduct the three-dimensional (3D) imaging of the heart beating in zebrafish larva at over 95 Hz with a low light dose.ResultsWe demonstrate that this approach reduces computational resources, brings a tenfold speedup, and achieves a tenfold improvement for the noise robustness in terms of SSIM over the state-of-the-art approach.ConclusionsWe proposed a phase-space deconvolution algorithm for 3D reconstructions in fluorescence imaging. Compared with the state-of-the-art method, we show significant improvement in both computational effectiveness and noise robustness; we further demonstrated practical application on zebrafish larva with low exposure and low light dose.

| S-EPMC9319196 | biostudies-literature

Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array.

Project description:Parallelized fluorescence imaging has been a long-standing pursuit that can address the unmet need for a comprehensive three-dimensional (3D) visualization of dynamical biological processes with minimal photodamage. However, the available approaches are limited to incomplete parallelization in only two dimensions or sparse sampling in three dimensions. We hereby develop a novel fluorescence imaging approach, called coded light-sheet array microscopy (CLAM), which allows complete parallelized 3D imaging without mechanical scanning. Harnessing the concept of an "infinity mirror", CLAM generates a light-sheet array with controllable sheet density and degree of coherence. Thus, CLAM circumvents the common complications of multiple coherent light-sheet generation in terms of dedicated wavefront engineering and mechanical dithering/scanning. Moreover, the encoding of multiplexed optical sections in CLAM allows the synchronous capture of all sectioned images within the imaged volume. We demonstrate the utility of CLAM in different imaging scenarios, including a light-scattering medium, an optically cleared tissue, and microparticles in fluidic flow. CLAM can maximize the signal-to-noise ratio and the spatial duty cycle, and also provides a further reduction in photobleaching compared to the major scanning-based 3D imaging systems. The flexible implementation of CLAM regarding both hardware and software ensures compatibility with any light-sheet imaging modality and could thus be instrumental in a multitude of areas in biological research.

| S-EPMC6971027 | biostudies-literature

Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy.

Project description:The single-photon timing and sensitivity performance and the imaging ability of asynchronous-readout single-photon avalanche diode (SPAD) array detectors have opened up enormous perspectives in fluorescence (lifetime) laser scanning microscopy (FLSM), such as super-resolution image scanning microscopy and high-information content fluorescence fluctuation spectroscopy. However, the strengths of these FLSM techniques depend on the many different characteristics of the detector, such as dark noise, photon-detection efficiency, after-pulsing probability, and optical cross talk, whose overall optimization is typically a trade-off between these characteristics. To mitigate this trade-off, we present, to our knowledge, a novel SPAD array detector with an active cooling system that substantially reduces the dark noise without significantly deteriorating any other detector characteristics. In particular, we show that lowering the temperature of the sensor to -15°C significantly improves the signal/noise ratio due to a 10-fold decrease in the dark count rate compared with room temperature. As a result, for imaging, the laser power can be decreased by more than a factor of three, which is particularly beneficial for live-cell super-resolution imaging, as demonstrated in fixed and living cells expressing green-fluorescent-protein-tagged proteins. For fluorescence fluctuation spectroscopy, together with the benefit of the reduced laser power, we show that cooling the detector is necessary to remove artifacts in the correlation function, such as spurious negative correlations observed in the hot elements of the detector, i.e., elements for which dark noise is substantially higher than the median value. Overall, this detector represents a further step toward the integration of SPAD array detectors in any FLSM system.

| S-EPMC8651514 | biostudies-literature

WIDE-FIELD SMARTPHONE FUNDUS VIDEO CAMERA BASED ON MINIATURIZED INDIRECT OPHTHALMOSCOPY.

Project description: Not available

| S-EPMC5777875 | biostudies-literature