Ontology highlight
ABSTRACT:
SUBMITTER: Velazquez G
PROVIDER: S-EPMC4615813 | biostudies-other | 2015
REPOSITORIES: biostudies-other
Velazquez Gilberto G Sousa Rui R Brieba Luis G LG
RNA biology 20150101 5
Single subunit RNA polymerases have evolved 2 mechanisms to synthesize long transcripts without falling off a DNA template: binding of nascent RNA and interactions with an RNA:DNA hybrid. Mitochondrial RNA polymerases share a common ancestor with T-odd bacteriophage single subunit RNA polymerases. Herein we characterized the role of the thumb subdomain of the yeast mtRNA polymerase gene (RPO41) in complex stability, processivity, and fidelity. We found that deletion and point mutants of the thum ...[more]