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Molecular characterization of an operon required for pertussis toxin secretion.

ABSTRACT: Mutants of Bordetella pertussis which are defective in secretion of pertussis toxin were isolated and characterized. The region of the B. pertussis chromosome identified by mutagenesis as playing a role in transport of pertussis toxin was sequenced. Analysis of this region revealed eight open reading frames, seven of which predict a protein exhibiting homology with one of the VirB proteins of Agrobacterium tumefaciens, which are involved in the transport of the T-DNA molecule across bacterial and plant membranes. Thus a set of accessory proteins are most likely involved in the secretion of pertussis toxin, and these proteins appear to be members of a family of proteins involved in the secretion of macromolecules from bacteria.

SUBMITTER: Weiss AA

PROVIDER: S-EPMC46218 | biostudies-other | 1993 Apr

REPOSITORIES: biostudies-other

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Molecular characterization of an operon required for pertussis toxin secretion.

Weiss A A AA Johnson F D FD Burns D L DL

Proceedings of the National Academy of Sciences of the United States of America 19930401 7

Mutants of Bordetella pertussis which are defective in secretion of pertussis toxin were isolated and characterized. The region of the B. pertussis chromosome identified by mutagenesis as playing a role in transport of pertussis toxin was sequenced. Analysis of this region revealed eight open reading frames, seven of which predict a protein exhibiting homology with one of the VirB proteins of Agrobacterium tumefaciens, which are involved in the transport of the T-DNA molecule across bacterial an ...[more]

PMID: 8464913

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