Unknown

Dataset Information

0

Arteriole dilation to synaptic activation that is sub-threshold to astrocyte endfoot Ca2+ transients.

ABSTRACT: Ca(2+)-dependent pathways in neurons and astrocyte endfeet initiate changes in arteriole diameter to regulate local brain blood flow. Whether there exists a threshold of synaptic activity in which arteriole diameter is controlled independent of astrocyte endfeet Ca(2+) remains unclear. We used two-photon fluorescence microscopy to examine synaptically evoked synthetic or genetic Ca(2+) indicator signals around penetrating arterioles in acute slices of the rat neocortex. We discovered a threshold below which vasodilation occurred in the absence of endfeet Ca(2+) signals but with consistent neuronal Ca(2+) transients, suggesting endfoot Ca(2+) is not necessary for activity-dependent vasodilation under subtle degrees of brain activation.

SUBMITTER: Institoris A 

PROVIDER: S-EPMC4640329 | biostudies-other | 2015 Sep

REPOSITORIES: biostudies-other

Json Xml
altmetric image

Publications

Arteriole dilation to synaptic activation that is sub-threshold to astrocyte endfoot Ca2+ transients.

Institoris Ádám Á   Rosenegger David George DG   Gordon Grant Robert GR  

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 20150701 9

Ca(2+)-dependent pathways in neurons and astrocyte endfeet initiate changes in arteriole diameter to regulate local brain blood flow. Whether there exists a threshold of synaptic activity in which arteriole diameter is controlled independent of astrocyte endfeet Ca(2+) remains unclear. We used two-photon fluorescence microscopy to examine synaptically evoked synthetic or genetic Ca(2+) indicator signals around penetrating arterioles in acute slices of the rat neocortex. We discovered a threshold  ...[more]