Physiological and biochemical mechanisms associated with trehalose-induced copper-stress tolerance in rice.
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ABSTRACT:
SUBMITTER: Mostofa MG
PROVIDER: S-EPMC4650698 | biostudies-other | 2015
REPOSITORIES: biostudies-other
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Project description:Rice yield has decreased due to climate variability and change in Colombia. Plant growth regulators have been used as a strategy to mitigate heat stress in different crops. Therefore, this study aimed to evaluate the effect of foliar applications of four growth regulators [auxins (AUX), brassinosteroids (BR), cytokinins (CK), or gibberellins (GA)] on physiological (stomatal conductance, total chlorophyll content, Fv/Fm ratio, plant canopy temperature, and relative water content) and biochemical (Malondialdehyde (MDA) and proline contents) variables in two commercial rice genotypes exposed to combined heat stress (high day and nighttime temperatures). Two separate experiments were carried out using plants of two rice genotypes, Fedearroz 67 ("F67") and Fedearroz 2000 ("F2000") for the first and second experiments, respectively. Both trials were analyzed together as a series of experiments. The established treatments were as follows: absolute control (AC) (rice plants grown under optimal temperatures (30/25°C day/nighttime temperatures), heat stress control (SC) [rice plants only exposed to combined heat stress (40/30°C)], and stressed rice plants and sprayed twice (5 days before and after heat stress) with a plant growth regulator (stress+AUX, stress+BR, stress+CK, or stress+GA). The results showed that foliar CK sprays enhanced the total chlorophyll content in both cultivars (3.25 and 3.65 mg g-1 fresh weight for "F67" and "F2000" rice plants, respectively) compared to SC plants (2.36 and 2.56 mg g-1 fresh weight for "F67," and "F2000" rice plants, respectively). Foliar CK application also improved stomatal conductance mainly in "F2000" rice plants compared to their heat stress control (499.25 vs.150.60 mmol m-2s-1). Foliar BR or CK sprays reduced plant canopy temperature between 2 and 3°C and MDA content in plants under heat stress. The relative tolerance index suggested that foliar CK (97.69%), and BR (60.73%) applications helped to mitigate combined heat stress mainly in "F2000" rice plants. In conclusion, foliar BR or CK applications can be considered an agronomic strategy to help to ameliorate the negative effect of combined heat stress conditions on the physiological behavior of rice plants.
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Project description:IntroductionArsenic (As) contamination in soil, sediments, and water poses a significant threat to the growth of bamboo plants. However, nanoparticles with high metal absorbance capacity can play a key role in the reduction of heavy metals toxicity in plants as well as maintaining their growth under toxicity.MethodsHence, an in vitro experiment was conducted to determine the influence of three types of nanoparticles: 150 µM silicon nanoparticles (SiO2 NPs), 150 µM titanium nanoparticles (TiO2 NPs), and 150 µM zinc oxide nanoparticles (ZnO NPs) on As (150 µM and 250 µM) tolerance enhancement of a one-year-old bamboo species (Pleioblastus pygmaeus).Results and discussionThe results showed that while As at 150 µM and 250 µM significantly disrupted the plant growth by excessive generation of reactive oxygen species (ROS) components, and inducing cell membrane peroxidation, the addition of NPs increased both enzymatic and non-enzymatic antioxidant activities, upregulated glyoxalase defense system, and improved gas exchange parameters and photosynthetic pigments content, leading to the enhanced plant shoot and root dry weight. These were achieved by lowering levels of ROS, electrolyte leakage (EL), malondialdehyde (MDA), hydrogen peroxide (H2O2) and the superoxide radical ( O2•- ), as well as decreasing As accumulation in the plant organs. Thus, it might be concluded that ZnO NPs, SiO2NPs, and TiO2NPS alone or in combination can significantly increase the bamboo plant tolerance to As toxicity via key mechanisms, including induction of various antioxidants and glyoxalase defense systems, scavenging of ROS and methylglyoxal (MG), increasing phytochelatins production, reduction of As accumulation and translocation, and improving photosynthetic pigments under As toxicity. Additionally, the results showed that the combined application of 150 µM ZnO NPs, SiO2 NPs, and TiO2 NPs had the greatest effect on enhancing the plant tolerance to As at 150 µM and 250 µM.
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Project description:Salinity is one of the most severe abiotic stress in the world. Also, the irrigated lands have been treated with second salinity. Canola is one of the most important industrial crops for oil production all over the world which is affected by salinity. Salt stress causes imbalanced ion hemostasis (Na+ and K+) and interrupted mineral absorption in canola. Also, salinity stress leads to oxidative stress (production and accumulation of reactive oxygen species (ROS). Accumulation of ROS is extremely dangerous and lethal for plants. As a consequence, canola production is reduced under salinity stress. So, a suitable approach should be found to deal with salinity stress and prevent the loss of production oilseed. Plant growth-promoting rhizobacteria (PGPR) can colonize on the plant root surface and alleviate the salt stress effect by providing minerals like nitrogen, phosphate, and potassium. Also, they alleviate salt stress by phytohormones like auxin (IAA), cytokinin (CK), and abscisic acid (ABA). This study focus on physiological parameters like leaf area (LA), root length (RL), shoot length (SL), chlorophyll fluorescence indexes (Fv/Fm and Fv/F0), relative water content (RWC), electrolyte leakage index (ELI), photosynthesis pigments (chlorophyll a, b, and carotenoids), Na+, and K+; and biochemical parameters like malondialdehyde (MDA) content, hydrogen peroxide content (H2O2), total protein content, proline, antioxidant capacity, and antioxidant enzyme activities in canola through the inoculation with Enterobacter sp. S16-3 and Pseudomonas sp. C16-2O. This study showed that LA, RL, SL, chlorophyll fluorescence indexes, RWC were significantly increased and ELI was significantly decreased in bacteria inoculated treatments. Also, MDA, H2O2 were decreased, and antioxidant capacity, proline, and antioxidant enzymes were increased due to inoculation with these bacteria. Besides, the amount of K+ as an index of salinity tolerance significantly increased, and leaf Na+ content was significantly decreased.
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Project description:BackgroundCadmium (Cd) pollution of soils is a global concern because its accumulation in plants generates severe growth retardation and health problems. Hibiscus syriacus is an ornamental plant that can tolerate various abiotic stresses, including Cd stress. Therefore, it is proposed as a plant material in Cd-polluted areas. However, the molecular mechanisms of H. syriacus tolerance to Cd are not yet understood.ResultsThis study investigated the physiological and transcriptional response of "Hongxing", a Cd2+-tolerant H. syriacus variety, grown on a substrate containing higher concentration of Cd (400 mg/kg). The Cd treatment induced only 28% of plant mortality, but a significant decrease in the chlorophyll content was observed. Malondialdehyde content and activity of the antioxidant enzymes catalase, peroxidase, and superoxide dismutase were significantly increased under Cd stress. Transcriptome analysis identified 29,921 differentially expressed genes (DEGs), including 16,729 down-regulated and 13,192 up-regulated genes, under Cd stress. Functional enrichment analyses assigned the DEGs mainly to plant hormone signal transduction, transport, nucleosome and DNA processes, mitogen-activated protein kinase signaling pathway, antioxidant process, fatty acid metabolism, and biosynthesis of secondary metabolites. Many MYB, EP2/ERF, NAC, WRKY family genes, and genes containing metal binding domains were up-regulated, implying that they are essential for the Cd-stress response in H. syriacus. The most induced genes were filtered out, providing valuable resources for future studies.ConclusionsOur findings provide insights into the molecular responses to Cd stress in H. syriacus. Moreover, this study offers comprehensive and important resources for future studies toward improving the plant Cd tolerance and its valorization in phytoremediation.
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