Surface vs. solution hybridization: effects of salt, temperature, and probe type.
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ABSTRACT:
SUBMITTER: Qiao W
PROVIDER: S-EPMC4659738 | biostudies-other | 2015 Dec
REPOSITORIES: biostudies-other
Publications
Surface vs. solution hybridization: effects of salt, temperature, and probe type.
Qiao Wanqiong W Chiang Hao-Chun HC Xie Hui H Levicky Rastislav R
Chemical communications (Cambridge, England) 20151201 97
Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior. ...[more]
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