Unknown

Dataset Information

0

Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers.


ABSTRACT: Au- and iron-based magnetic nanoparticles (NPs) are promising NPs for biomedical applications due to their unique properties. The combination of a gold coating over a magnetic core puts together the benefits from adding the magnetic properties to the robust chemistry provided by the thiol functionalization of gold. Here, the use of Au-coated magnetic NPs for molecular detection of a single methylation in DNA aptamer is described. Binding of ?-thrombin to two aptamers conjugated to these NPs causes aggregation, a phenomenon that can be observed by UV, DLS and MRI. These techniques discriminate a single methylation in one of the aptamers, preventing aggregation due to the inability of ?-thrombin to recognize it. A parallel study with gold and ferromagnetic NPs is detailed, concluding that the Au coating of FexOy NP does not affect their performance and that they are suitable as complex biosensors. These results prove the high detection potency of Au-coated SPIONs for biomedical applications especially for DNA repair detection.

SUBMITTER: Tintore M 

PROVIDER: S-EPMC4661904 | biostudies-other | 2015

REPOSITORIES: biostudies-other

altmetric image

Publications

Gold-Coated Superparamagnetic Nanoparticles for Single Methyl Discrimination in DNA Aptamers.

Tintoré Maria M   Mazzini Stefania S   Polito Laura L   Marelli Marcello M   Latorre Alfonso A   Somoza Álvaro Á   Aviñó Anna A   Fàbrega Carme C   Eritja Ramon R  

International journal of molecular sciences 20151118 11


Au- and iron-based magnetic nanoparticles (NPs) are promising NPs for biomedical applications due to their unique properties. The combination of a gold coating over a magnetic core puts together the benefits from adding the magnetic properties to the robust chemistry provided by the thiol functionalization of gold. Here, the use of Au-coated magnetic NPs for molecular detection of a single methylation in DNA aptamer is described. Binding of α-thrombin to two aptamers conjugated to these NPs caus  ...[more]

Similar Datasets

| S-EPMC6186064 | biostudies-literature
| S-EPMC11240255 | biostudies-literature
2022-09-01 | GSE197944 | GEO
| S-EPMC6404081 | biostudies-literature
| S-EPMC8070144 | biostudies-literature
2018-01-05 | GSE93187 | GEO
| S-EPMC4289153 | biostudies-literature
| S-EPMC9083275 | biostudies-literature
| S-EPMC4271257 | biostudies-other