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HisAK70: progress towards a vaccine against different forms of leishmaniosis.

ABSTRACT: Leishmania major and Leishmania infantum are among the main species that are responsible for cutaneous leishmaniosis (CL) and visceral leishmaniosis (VL), respectively. The leishmanioses represent the second-largest parasitic killer in the world after malaria. Recently, we succeeded in generating a plasmid DNA (pCMV-HISA70m2A) and demonstrated that immunized mice were protected against L. major challenge. The efficacy of the DNA-vaccine was further enhanced by the inclusion of KMP-11 antigen into the antibiotic-free plasmid pVAX1-asd.Here, we describe the use of a HisAK70 DNA-vaccine encoding seven Leishmania genes (H2A, H2B, H3, H4, A2, KMP11 and HSP70) for vaccination of mice to assess the induction of a resistant phenotype against VL and CL.HisAK70 was successful in vaccinated mice, resulting in a high amount of efficient sterile hepatic granulomas associated with a hepatic parasite burden fully resolved in the VL model; and resulting in 100% inhibition of parasite visceralization in the CL model.The results suggest that immunization with the HisAK70 DNA-vaccine may provide a rapid, suitable, and efficient vaccination strategy to confer cross-protective immunity against VL and CL.

SUBMITTER: Dominguez-Bernal G

PROVIDER: S-EPMC4675018 | biostudies-other | 2015

REPOSITORIES: biostudies-other

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HisAK70: progress towards a vaccine against different forms of leishmaniosis.

Domínguez-Bernal Gustavo G Horcajo Pilar P Orden José A JA Ruiz-Santa-Quiteria José A JA De La Fuente Ricardo R Ordóñez-Gutiérrez Lara L Martínez-Rodrigo Abel A Mas Alicia A Carrión Javier J

Parasites & vectors 20151209

<h4>Background</h4>Leishmania major and Leishmania infantum are among the main species that are responsible for cutaneous leishmaniosis (CL) and visceral leishmaniosis (VL), respectively. The leishmanioses represent the second-largest parasitic killer in the world after malaria. Recently, we succeeded in generating a plasmid DNA (pCMV-HISA70m2A) and demonstrated that immunized mice were protected against L. major challenge. The efficacy of the DNA-vaccine was further enhanced by the inclusion of ...[more]

PMID: 26653170

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Dataset Information

HisAK70: progress towards a vaccine against different forms of leishmaniosis.

Publications

HisAK70: progress towards a vaccine against different forms of leishmaniosis.

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