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3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo.

ABSTRACT: We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in brain tissue in vivo. Adaptive pipette positioning provides a platform for future advances in automated, single cell in vivo experiments.

SUBMITTER: Long B 

PROVIDER: S-EPMC4686883 | biostudies-other | 2015 Dec

REPOSITORIES: biostudies-other

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3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo.

Long Brian B   Li Lu L   Knoblich Ulf U   Zeng Hongkui H   Peng Hanchuan H  

Scientific reports 20151222

We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in brain tissue in vivo. Adaptive pipette positioning provides a platform for future advances in automated, single cell in vivo experiments. ...[more]

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