Dataset Information

Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium.

ABSTRACT: The International Knockout Mouse Consortium (IKMC) introduces its targeted constructs into C57BL/6N embryonic stem cells. However, breeding with a Cre-recombinase and/or Flp-recombinase mouse is required for the generation of a null allele with the IKMC cassette. Many recombinase strains are in the C57BL/6J background, resulting in knockout animals on a mixed strain background. This can lead to variability in metabolic data and the use of improper control groups. While C57BL/6N and C57BL/6J are derived from the same parental C57BL/6 strain, there are key genotypic and phenotypic differences between these substrains. Many researchers may not even be aware of these differences, as the shorthand C57BL/6 is often used to describe both substrains. We found that 58% of articles involving genetically modified mouse models did not completely address background strain. This review will describe these two substrains and highlight the importance of separate consideration in mouse model development. Our aim is to increase awareness of this issue in the diabetes research community and to provide practical strategies to enable researchers to avoid mixed strain animals when using IKMC knockout mice.

SUBMITTER: Fontaine DA

PROVIDER: S-EPMC4686949 | biostudies-other | 2016 Jan

REPOSITORIES: biostudies-other

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Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium.

Fontaine Danielle A DA Davis Dawn Belt DB

Diabetes 20160101 1

The International Knockout Mouse Consortium (IKMC) introduces its targeted constructs into C57BL/6N embryonic stem cells. However, breeding with a Cre-recombinase and/or Flp-recombinase mouse is required for the generation of a null allele with the IKMC cassette. Many recombinase strains are in the C57BL/6J background, resulting in knockout animals on a mixed strain background. This can lead to variability in metabolic data and the use of improper control groups. While C57BL/6N and C57BL/6J are ...[more]

PMID: 26696638

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Project description:Background: The Strengthening Capacity in Environmental Physics, Hydrogeology and Statistics for conservation agriculture research (CEPHaS) consortium sought to to strengthen research capacity among a network of African and UK researchers, and their respective institutions, to fill knowledge gaps on the impacts of conservation agriculture practices on the water cycle in cultivated soils. We examined experiences of consortium membership and, drawing on this information, determined key recommendations for future programmes with similar objectives. Methods: A mixed methods study encompassing an online survey (N=40) and semi-structured interviews (N=19) completed between June 2021 and February 2022 with CEPHaS consortium members from Malawi, UK, Zambia and Zimbabwe. Survey and interview data were analysed separately, using univariate statistics and framework synthesis respectively Results: Survey and interview findings were generally aligned, with both revealing a wide range of reported capacity strengthening gains resulting from CEPHaS engagement at both an individual and institutional level. Participants consistently expressed their CEPHaS involvement in positive terms with praise for the applied 'learn by doing' approach underpinning many of the activities as well as the engaging and highly inclusive leadership. There was evidence that the various trainings and resources provided through CEPHaS were valued, frequently utilised, and often transferred beyond the immediate CEPHaS membership for wider benefit. Resource provision and staff training were seen as foundational for long-term institutional benefits. Some challenges and suggested areas for improvement were reported by participants as were potential opportunities to facilitate greater impact. Conclusion: Our findings suggest that the basic 'template' of the CEPHaS consortium provided a strong basis for research capacity strengthening in Conservation Agriculture, especially at the level of individual researchers, and that this template could be further enhanced in any future iteration of the same or similar programme. Recommendations for replicating and enhancing CEPHaS programme strengths are presented.

Dataset Information

Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium.

Publications

Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium.

Similar Datasets

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