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Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity.

ABSTRACT: Analysis of embryonic fibroblasts from GFP reporter mice indicates that the fibroblast cell type harbors a large collection of developmentally and phenotypically heterogeneous subtypes. Some of these cells exhibit multipotency, whereas others do not. Multiparameter flow cytometry analysis shows that a large number of distinct populations of fibroblast-like cells can be found in cultures initiated from different embryonic organs, and cells sorted according to their surface phenotype typically retain their characteristics on continued propagation in culture. Similarly, surface phenotypes of individual cloned fibroblast-like cells exhibit significant variation. The fibroblast cell class appears to contain a very large number of denumerable subtypes.

SUBMITTER: Singhal PK

PROVIDER: S-EPMC4711836 | biostudies-other | 2016 Jan

REPOSITORIES: biostudies-other

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Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity.

Singhal Prabhat K PK Sassi Slim S Lan Lan L Au Patrick P Halvorsen Stefan C SC Fukumura Dai D Jain Rakesh K RK Seed Brian B

Proceedings of the National Academy of Sciences of the United States of America 20151222 1

Analysis of embryonic fibroblasts from GFP reporter mice indicates that the fibroblast cell type harbors a large collection of developmentally and phenotypically heterogeneous subtypes. Some of these cells exhibit multipotency, whereas others do not. Multiparameter flow cytometry analysis shows that a large number of distinct populations of fibroblast-like cells can be found in cultures initiated from different embryonic organs, and cells sorted according to their surface phenotype typically ret ...[more]

PMID: 26699463

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