In vitro analysis of Ah receptor domains involved in ligand-activated DNA recognition.
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ABSTRACT:
SUBMITTER: Dolwick KM
PROVIDER: S-EPMC47398 | biostudies-other | 1993 Sep
REPOSITORIES: biostudies-other
Publications
In vitro analysis of Ah receptor domains involved in ligand-activated DNA recognition.
Dolwick K M KM Swanson H I HI Bradfield C A CA
Proceedings of the National Academy of Sciences of the United States of America 19930901 18
The Ah receptor (AHR) is a basic helix-loop-helix protein that mediates the effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin. In this report, we describe a rabbit reticulocyte system that allows functional expression of both the AHR and its dimeric partner, the AHR nuclear translocator protein (ARNT). By using this in vitro system, we were able to reconstitute agonist binding to the AHR and agonist-induced AHR-ARNT recognition of a cognate DNA enhancer sequence. Expression of AHR deletion mutants ...[more]
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