Ontology highlight
ABSTRACT:
SUBMITTER: Dominik PK
PROVIDER: S-EPMC4740257 | biostudies-other | 2016 Feb
REPOSITORIES: biostudies-other
Dominik Pawel K PK Borowska Marta T MT Dalmas Olivier O Kim Sangwoo S SS Perozo Eduardo E Keenan Robert J RJ Kossiakoff Anthony A AA
Structure (London, England : 1993) 20151231 2
A major challenge in membrane biophysics is to define the mechanistic linkages between a protein's conformational transitions and its function. We describe a novel approach to stabilize transient functional states of membrane proteins in native-like lipid environments allowing for their structural and biochemical characterization. This is accomplished by combining the power of antibody Fab-based phage display selection with the benefits of embedding membrane protein targets in lipid-filled nanod ...[more]