Ontology highlight
ABSTRACT:
SUBMITTER: Wang L
PROVIDER: S-EPMC4751836 | biostudies-other | 2015 Dec
REPOSITORIES: biostudies-other
Applied and environmental microbiology 20151211 4
We developed a counterselectable deletion system for Thermus thermophilus HB27 based on cytosine deaminase (encoded by codA) from Thermaerobacter marianensis DSM 12885 and the sensitivity of T. thermophilus HB27 to the antimetabolite 5-fluorocytosine (5-FC). The deletion vector comprises the pUC18 origin of replication, a thermostable kanamycin resistance marker functional in T. thermophilus HB27, and codA under the control of a constitutive putative trehalose promoter from T. thermophilus HB27. ...[more]