Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cobalt oxide nanoparticles can enter inside the cells by crossing plasma membranes.

ABSTRACT: The ability of nanoparticles (NPs) to be promptly uptaken by the cells makes them both dangerous and useful to human health. It was recently postulated that some NPs might cross the plasma membrane also by a non-endocytotic pathway gaining access to the cytoplasm. To this aim, after having filled mature Xenopus oocytes with Calcein, whose fluorescence is strongly quenched by divalent metal ions, we have exposed them to different cobalt NPs quantifying quenching as evidence of the increase of the concentration of Co(2+) released by the NPs that entered into the cytoplasm. We demonstrated that cobalt oxide NPs, but not cobalt nor cobalt oxide NPs that were surrounded by a protein corona, can indeed cross plasma membranes.

SUBMITTER: Bossi E

PROVIDER: S-EPMC4770291 | biostudies-other | 2016 Feb

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Iron oxide nanoparticles can cross plasma membranes.

Project description:Iron deficiency is a major global public health problem despite decades of efforts with iron supplementation and fortification. The issue lies on the poor tolerability of the standard of care soluble iron salts, leading to non-compliance and ineffective correction of iron-deficiency anaemia. Iron nanoformulations have been proposed to fortify food and feed to address these issues. Since it was just postulated that some nanoparticles (NPs) might cross the plasma membrane also by a non-endocytotic pathway gaining direct access to the cytoplasm, we have studied iron NP uptake under this perspective. To this aim, we have used a recently tested protocol that has proven to be capable of following the cytoplasmic changes of iron concentration dynamics and we have demonstrated that iron oxide NPs, but not zerovalent iron NPs nor iron oxide NPs that were surrounded by a protein corona, can cross plasma membranes. By electrophysiology, we have also shown that a small and transient increase of membrane conductance parallels NP crossing of plasma membrane.

| S-EPMC5595914 | biostudies-literature

Mechanochemical Synthesis of Iron and Cobalt Magnetic Metal Nanoparticles and Iron/Calcium Oxide and Cobalt/Calcium Oxide Nanocomposites.

Project description:We report an environmentally benign and cost-effective method to produce Fe and Co magnetic metal nanoparticles as well as the Fe/Cao and Co/CaO nanocomposites by using a novel, dry mechanochemical process. Mechanochemical milling of metal oxides with a suitable reducing agent resulted in the production of magnetic metal nanoparticles. The process involved grinding and consequent reduction of low-costing oxide powders, unlike conventional processing techniques involving metal salts or metal complexes. Calcium granules were used as the reducing agent. Magnetometry measurements were performed over a large range of temperatures, from 10 to 1273 K, to evaluate the Curie temperature, blocking temperature, irreversibility temperature, saturation magnetization, and coercivity. The saturation magnetizations of the iron and cobalt nanoparticles were found to be 191 and 102 emu g-1, respectively. The heating abilities of these nanoparticles suspended in several liquids under alternating magnetic fields were measured and the specific loss power was determined. Our results suggest that the dry mechanochemical process is a robust method to produce metallic nanoparticles and nanocomposites.

| S-EPMC6080568 | biostudies-literature

Oxidation of Benzyl Alcohol Using Cobalt Oxide Supported Inside and Outside Hollow Carbon Spheres.

Project description:Cobalt oxide nanoparticles (6 nm) supported both inside and outside of hollow carbon spheres (HCSs) were synthesized by using two different polymer templates. The oxidation of benzyl alcohol was used as a model reaction to evaluate the catalysts. PXRD studies indicated that the Co oxidation state varied for the different catalysts due to reduction of the Co by the carbon, and a metal oxidation step prior to the benzyl alcohol oxidation enhanced the catalytic activity. The metal loading influenced the catalytic efficiency, and the activity decreased with increasing metal loading, possibly due to pore filling effects. The catalysts showed similar activity and selectivity (to benzaldehyde) whether placed inside or outside the HCS (63 % selectivity at 50 % conversion). No poisoning was observed due to product build up in the HCS.

| S-EPMC8173001 | biostudies-literature

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes.

Project description:BackgroundThe purpose of this work is to provide experimental evidence on the interactions of suspended nanoparticles with artificial or biological membranes and to assess the possibility of suspended nanoparticles interacting with the lipid component of biological membranes.Methods1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid vesicles and human red blood cells were incubated in suspensions of magnetic bare cobalt ferrite (CoFe2O4) or citric acid (CA)-adsorbed CoFe2O4 nanoparticles dispersed in phosphate-buffered saline and glucose solution. The stability of POPC giant unilamellar vesicles after incubation in the tested nanoparticle suspensions was assessed by phase-contrast light microscopy and analyzed with computer-aided imaging. Structural changes in the POPC multilamellar vesicles were assessed by small angle X-ray scattering, and the shape transformation of red blood cells after incubation in tested suspensions of nanoparticles was observed using scanning electron microscopy and sedimentation, agglutination, and hemolysis assays.ResultsArtificial lipid membranes were disturbed more by CA-adsorbed CoFe2O4 nanoparticle suspensions than by bare CoFe2O4 nanoparticle suspensions. CA-adsorbed CoFe2O4-CA nanoparticles caused more significant shape transformation in red blood cells than bare CoFe2O4 nanoparticles.ConclusionConsistent with their smaller sized agglomerates, CA-adsorbed CoFe2O4 nanoparticles demonstrate more pronounced effects on artificial and biological membranes. Larger agglomerates of nanoparticles were confirmed to be reactive against lipid membranes and thus not acceptable for use with red blood cells. This finding is significant with respect to the efficient and safe application of nanoparticles as medicinal agents.

| S-EPMC3970951 | biostudies-literature

Human keratinocytes exposed to tungsten carbide and tungsten carbide cobalt nanoparticles and cobalt chloride

Project description:Comparison of transcriptional profiles after exposure of HaCaT cells to WC or WC-Co nanoparticles and respective amount of free cobalt in form of CoCl2 after 3 hours and 3 days of exposure. Genes responding especially to nanoparticles or leached cobalt ions should be determined.

2010-02-01 | GSE16727 | GEO

Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes.

Project description:It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show that noncytotoxic concentrations of cationic nanoparticles induce 30-2000 pA currents in 293A (human embryonic kidney) and KB (human epidermoid carcinoma) cells, consistent with a nanoscale defect such as a single hole or group of holes in the cell membrane ranging from 1 to 350 nm(2) in total area. Other forms of nanoscale defects, including the nanoparticle porating agents adsorbing onto or intercalating into the lipid bilayer, are also consistent; although the size of the defect must increase to account for any reduction in ion conduction, as compared to a water channel. An individual defect forming event takes 1-100 ms, while membrane resealing may occur over tens of seconds. Patch-clamp data provide direct evidence for the formation of nanoscale defects in living cell membranes. The cationic polymer data are compared and contrasted with patch-clamp data obtained for an amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-3), a small molecule that is proposed to make well-defined 3.4 nm holes in lipid bilayers. Here, we observe data that are consistent with AMO-3 making approximately 3 nm holes in living cell membranes.

| S-EPMC2753177 | biostudies-literature

Interaction of iron oxide nanoparticles with human plasma

Project description:The Interaction of iron oxide nanoparticles with human plasma was studied.

2019-04-11 | PXD004441 | Pride

Laser Fragmentation-Induced Defect-Rich Cobalt Oxide Nanoparticles for Electrochemical Oxygen Evolution Reaction.

Project description:Sub-5?nm cobalt oxide nanoparticles are produced in a flowing water system by pulsed laser fragmentation in liquid (PLFL). Particle fragmentation from 8?nm to 4?nm occurs and is attributed to the oxidation process in water where oxidative species are present and the local temperature is rapidly elevated under laser irradiation. Significantly higher surface area, crystal phase transformation, and formation of structural defects (Co2+ defects and oxygen vacancies) through the PLFL process are evidenced by detailed structural characterizations by nitrogen physisorption, electron microscopy, synchrotron X-ray diffraction, and X-ray photoelectron spectroscopy. When employed as electrocatalysts for the oxygen evolution reaction under alkaline conditions, the fragmented cobalt oxides exhibit superior catalytic activity over pristine and nanocast cobalt oxides, delivering a current density of 10?mA?cm-2 at 369?mV and a Tafel slope of 46?mV?dec-1 , which is attributed to a larger exposed active surface area, the formation of defects, and an increased charge transfer rate. The study provides an effective approach to engineering cobalt oxide nanostructures in a flowing water system, which shows great potential for sustainable production of active cobalt catalysts.

| S-EPMC7028056 | biostudies-literature

Optical micromanipulation of nanoparticles and cells inside living zebrafish.

Project description:Regulation of biological processes is often based on physical interactions between cells and their microenvironment. To unravel how and where interactions occur, micromanipulation methods can be used that offer high-precision control over the duration, position and magnitude of interactions. However, lacking an in vivo system, micromanipulation has generally been done with cells in vitro, which may not reflect the complex in vivo situation inside multicellular organisms. Here using optical tweezers we demonstrate micromanipulation throughout the transparent zebrafish embryo. We show that different cells, as well as injected nanoparticles and bacteria can be trapped and that adhesion properties and membrane deformation of endothelium and macrophages can be analysed. This non-invasive micromanipulation inside a whole-organism gives direct insights into cell interactions that are not accessible using existing approaches. Potential applications include screening of nanoparticle-cell interactions for cancer therapy or tissue invasion studies in cancer and infection biology.

| S-EPMC4802177 | biostudies-literature

Accessible Silver-Iron Oxide Nanoparticles as a Nanomaterial for Supported Liquid Membranes.

Project description:The present study introduces the process performances of nitrophenols pertraction using new liquid supported membranes under the action of a magnetic field. The membrane system is based on the dispersion of silver-iron oxide nanoparticles in n-alcohols supported on hollow microporous polypropylene fibers. The iron oxide-silver nanoparticles are obtained directly through cyclic voltammetry electrolysis run in the presence of soluble silver complexes ([AgCl2]-; [Ag(S2O3)2]3-; [Ag(NH3)2]+) and using pure iron electrodes. The nanostructured particles are characterized morphologically and structurally by scanning electron microscopy (SEM and HFSEM), EDAX, XRD, and thermal analysis (TG, DSC). The performances of the nitrophenols permeation process are investigated in a variable magnetic field. These studies show that the flux and extraction efficiency have the highest values for the membrane system embedding iron oxide-silver nanoparticles obtained electrochemically in the presence of [Ag(NH3)2]+ electrolyte. It is demonstrated that the total flow of nitrophenols through the new membrane system depends on diffusion, convection, and silver-assisted transport.

| S-EPMC8147404 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data