Direct spectrophotometric measurement of supra-physiological levels of ascorbate in plasma.
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ABSTRACT:
SUBMITTER: Witmer JR
PROVIDER: S-EPMC4776628 | biostudies-other | 2016 Aug
REPOSITORIES: biostudies-other
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Direct spectrophotometric measurement of supra-physiological levels of ascorbate in plasma.
Witmer Jordan R JR Wetherell Bailey J BJ Wagner Brett A BA Du Juan J Cullen Joseph J JJ Buettner Garry R GR
Redox biology 20160213
<h4>Background</h4>Supra-physiological concentrations of ascorbate, vitamin C, in blood, greater than 1mM, achieved through intravenous administration (IV), are being tested in clinical trials to treat human disease, e.g. cancer. These trials need information on the high levels of ascorbate achieved in blood upon IV administration of pharmacological ascorbate so appropriate clinical decisions can be made.<h4>Methods</h4>Here we demonstrate that in the complex matrix of human blood plasma supra-p ...[more]
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