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Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.

ABSTRACT: In a screen designed to isolate human cDNAs that complement a yeast G2 phase checkpoint mutation (mec1), we isolated a cDNA homologous to the Saccharomyces cerevisiae CDC34 gene. The human CDC34 cDNA can functionally substitute for the yeast CDC34 gene and represents a mammalian homolog of the group of yeast genes required for the late G1-->S phase transition. The human CDC34 gene is expressed in multiple cell lines as a unique species and Southern blot analysis reveals evidence for a single gene that is highly conserved in higher eukaryotes. The human gene is located on the far telomeric region of 19p13.3 in a location that defines a region of homology between human chromosome 19p and mouse chromosome 11.

SUBMITTER: Plon SE

PROVIDER: S-EPMC47801 | biostudies-other | 1993 Nov

REPOSITORIES: biostudies-other

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Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.

Plon S E SE Leppig K A KA Do H N HN Groudine M M

Proceedings of the National Academy of Sciences of the United States of America 19931101 22

In a screen designed to isolate human cDNAs that complement a yeast G2 phase checkpoint mutation (mec1), we isolated a cDNA homologous to the Saccharomyces cerevisiae CDC34 gene. The human CDC34 cDNA can functionally substitute for the yeast CDC34 gene and represents a mammalian homolog of the group of yeast genes required for the late G1-->S phase transition. The human CDC34 gene is expressed in multiple cell lines as a unique species and Southern blot analysis reveals evidence for a single gen ...[more]

PMID: 8248134

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