Establishment of a cone photoreceptor transplantation platform based on a novel cone-GFP reporter mouse line.
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ABSTRACT:
SUBMITTER: Smiley S
PROVIDER: S-EPMC4786810 | biostudies-other | 2016 Mar
REPOSITORIES: biostudies-other
Publications
Establishment of a cone photoreceptor transplantation platform based on a novel cone-GFP reporter mouse line.
Smiley Sheila S Nickerson Philip E PE Comanita Lacrimioara L Daftarian Narsis N El-Sehemy Ahmed A Tsai En Leh Samuel EL Matan-Lithwick Stuart S Yan Keqin K Thurig Sherry S Touahri Yacine Y Dixit Rajiv R Aavani Tooka T De Repentingy Yves Y Baker Adam A Tsilfidis Catherine C Biernaskie Jeff J Sauvé Yves Y Schuurmans Carol C Kothary Rashmi R Mears Alan J AJ Wallace Valerie A VA
Scientific reports 20160311
We report successful retinal cone enrichment and transplantation using a novel cone-GFP reporter mouse line. Using the putative cone photoreceptor-enriched transcript Coiled-Coil Domain Containing 136 (Ccdc136) GFP-trapped allele, we monitored developmental reporter expression, facilitated the enrichment of cones, and evaluated transplanted GFP-labeled cones in wildtype and retinal degeneration mutant retinas. GFP reporter and endogenous Ccdc136 transcripts exhibit overlapping temporal and spati ...[more]
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