Ontology highlight
ABSTRACT:
SUBMITTER: Nakatsugawa M
PROVIDER: S-EPMC4814874 | biostudies-other | 2016 Mar
REPOSITORIES: biostudies-other
Nakatsugawa Munehide M Rahman Muhammed A MA Yamashita Yuki Y Ochi Toshiki T Wnuk Piotr P Tanaka Shinya S Chamoto Kenji K Kagoya Yuki Y Saso Kayoko K Guo Tingxi T Anczurowski Mark M Butler Marcus O MO Hirano Naoto N
Scientific reports 20160331
Recent high throughput sequencing analysis has revealed that the TCRβ repertoire is largely different between CD8(+) and CD4(+) T cells. Here, we show that the transduction of SIG35α, the public chain-centric HLA-A*02:01(A2)/MART127-35 TCRα hemichain, conferred A2/MART127-35 reactivity to a substantial subset of both CD8(+) and CD4(+) T cells regardless of their HLA-A2 positivity. T cells individually reconstituted with SIG35α and different A2/MART127-35 TCRβ genes isolated from CD4(+) or CD8(+) ...[more]